Exome capture sequencing of extracted DNA was done by IDTxGen lockdown human panel (Admera Health, LLC South Plainfield, NJ).

Captured nucleotides were subjected to 150–base pair paired-end sequencing on an Illumina HiSeq platform (Admera Health, South Plainfield, NJ).

Exome sequencing was performed by Admera Health.

Whole-exome sequencing (WES) was performed by Admera Health.

For WES, tumor DNA samples from 16 formalin-fixed, paraffin-embedded (FFPE) tumors and germline DNA samples from four buffy coats were extracted and sequenced at Admera Health.

Whole-exome sequencing (WES) was performed with an Illumina HiSeq platform (Admera Health, New Jersey), aiming for a 90% coverage of the IDT Xgen library, human genome version 38 (hg38, December 2013)

Exome capture sequencing of extracted DNA was done by IDTxGen lockdown human panel (Admera Health, LLC South Plainfield, NJ).

Whole Genome Sequencing and Variant Filtering DNA collected from the affected calf, the dam, the sire, and three paternal half-siblings was sent to Admera Health (South Plainfield, NJ) for KAPA library prep and 150 bp paired-end sequencing on an Illumina NovaSeq to a targeted sequencing depth of 12X

Libraries were sequenced on an Illumina MiSeq 2 × 150 platform (San Diego, CA, USA) through the Admera Health Company (South Plainfield, NJ, USA).